SKA1

Gene symbol: SKA1
Gene: 220134
Uniprot Function: Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020). In the complex, it mediates the interaction with microtubules (PubMed:19289083, PubMed:23085020).
Mitotic Index 0.1673
Interphase Cluster: #95 (24 genes)
Mitotic Cluster: #11 (44 genes)
sgRNA 1: AATTTGATTATAGGTTAAGC (interphase cells: 287, mitotic cells: 35)
sgRNA 2: ACCTTATCATTAAGAAACTG (interphase cells: 440, mitotic cells: 101)
sgRNA 3: CATTACCTTGAGTGAATTGT (interphase cells: 335, mitotic cells: 103)
sgRNA 4: CTCTGGGTTACTGTTACTTG (interphase cells: 944, mitotic cells: 164)

Interphase Cluster

Mitotic Cluster

Overview

Each montage below shows randomly selected individual cell images for the same target gene separated by sgRNA, along with an example montage of cells expressing a non-targeting negative control sgRNA at the bottom. sgRNA labels in the montages correspond to the numbered sgRNA sequences in the gene info table above. The DNA (blue) and tubulin (green) channels are displayed by default, with gH2AX (grayscale) and actin (yellow) available using the check boxes. Channel brightness, contrast, and opacity are adjustable by clicking the arrow next to the channel name. When only one channel is selected, the display defaults to grayscale. Click the “Zoom” checkbox above the channel controls to enable image zooming (via scrolling) and panning (via click-and-drag).

The cell boxes displayed for this gene are square, 30.36 μm per side for the interphase montage and 30.36 μm per side for the mitotic montage.

Features

Interphase Score Rank FDR q-value
Nucleus DNA Integrated 0.067 681 of 5322 0.446
Nucleus DNA Mean 0.080 587 of 5322 0.143
Nucleus gH2AX Mean 0.071 2817 of 5322 0.181
Nucleus DNA - gH2AX Correlation 0.007 790 of 5322 0.932
Nucleus Shape Area 0.043 1889 of 5322 0.647
Nucleus Shape Eccentricity 0.130 88 of 5322 0.009
Nucleus Shape Form Factor -1.207e-01 4704 of 5322 0.023
Nucleus Shape Solidity -1.001e-01 4278 of 5322 0.037
Cell DNA Mass Displacement 0.057 865 of 5322 0.507
Cell Tubulin Mean 0.119 378 of 5322 0.022
Cell Tubulin Mass Displacement 0.022 1657 of 5322 0.834
Cell Actin Mean 0.016 2479 of 5322 0.846
Cell Tubulin - Actin Correlation 0.044 1626 of 5322 0.507
Cell DNA - Tubulin Correlation 0.126 663 of 5322 0.011
Cell Shape Area -9.414e-02 4767 of 5322 0.075
Cell Shape Eccentricity 0.049 599 of 5322 0.642
Mitotic Score Rank FDR q-value
Nucleus DNA Integrated 0.576 75 of 5322 0.064
Nucleus DNA Mean -2.813e-02 3406 of 5322 1.000
Nucleus DNA Mass Displacement 0.483 34 of 5322 0.999
Nucleus DNA Mean Frac 3 0.521 36 of 5322 0.090
Nucleus gH2AX Mean -2.246e-02 3248 of 5322 1.000
Nucleus gH2AX Mean Frac 0 0.267 156 of 5322 1.000
Nucleus Tubulin Mean Frac 0 0.564 63 of 5322 0.022
Nucleus Tubulin Mean Edge 5.674e-04 3171 of 5322 1.000
Nucleus Tubulin Std Edge -4.740e-01 5164 of 5322 0.024
Nucleus Shape Area 0.860 63 of 5322 0.001
Nucleus Shape Eccentricity 0.018 1823 of 5322 1.000
Nucleus Shape Form Factor -7.656e-01 5294 of 5322 0.049
Cell Tubulin Mean 0.730 26 of 5322 0.004
Cell Tubulin Mean Frac 3 -5.490e-01 5228 of 5322 0.022
Cell Actin Mean -2.409e-02 3542 of 5322 0.997
Cell Shape Area 0.336 530 of 5322 0.646
( Reset Zoom )
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity

( Reset Zoom )
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity
Brightness
Contrast
Opacity

Day 3

Day 4

MIT Central Accessability