CNOT10

Gene symbol: CNOT10
Gene: 25904
Uniprot Function: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.
Mitotic Index 0.0211
Interphase Cluster: #157 (18 genes)
Mitotic Cluster: #18 (40 genes)
sgRNA 1: AAGGTAGACTGCCAGCAAGA (interphase cells: 1092, mitotic cells: 19)
sgRNA 2: GCCAGCCGTAGCCAGAGGCG (interphase cells: 1130, mitotic cells: 30)
sgRNA 3: GCTGCATTGCTGCCAATAAG (interphase cells: 1370, mitotic cells: 26)
sgRNA 4: TTGGTTAGTAACGTACACAT (interphase cells: 919, mitotic cells: 22)

Interphase Cluster

Mitotic Cluster

Overview

Each montage below shows randomly selected individual cell images for the same target gene separated by sgRNA, along with an example montage of cells expressing a non-targeting negative control sgRNA at the bottom. sgRNA labels in the montages correspond to the numbered sgRNA sequences in the gene info table above. The DNA (blue) and tubulin (green) channels are displayed by default, with gH2AX (grayscale) and actin (yellow) available using the check boxes. Channel brightness, contrast, and opacity are adjustable by clicking the arrow next to the channel name. When only one channel is selected, the display defaults to grayscale. Click the “Zoom” checkbox above the channel controls to enable image zooming (via scrolling) and panning (via click-and-drag).

The cell boxes displayed for this gene are square, 30.36 μm per side for the interphase montage and 30.36 μm per side for the mitotic montage.

Features

Interphase Score Rank FDR q-value
Nucleus DNA Integrated 0.033 1565 of 5322 0.689
Nucleus DNA Mean -1.909e-02 2611 of 5322 0.744
Nucleus gH2AX Mean 0.240 422 of 5322 1.367e-04
Nucleus DNA - gH2AX Correlation -2.677e-01 5246 of 5322 1.021e-04
Nucleus Shape Area -1.991e-02 3889 of 5322 0.655
Nucleus Shape Eccentricity 0.085 313 of 5322 0.025
Nucleus Shape Form Factor -9.556e-02 4291 of 5322 0.016
Nucleus Shape Solidity -1.164e-01 4556 of 5322 8.893e-04
Cell DNA Mass Displacement 0.023 2876 of 5322 0.772
Cell Tubulin Mean 0.929 4 of 5322 2.141e-04
Cell Tubulin Mass Displacement 0.381 8 of 5322 3.984e-04
Cell Actin Mean 0.065 1266 of 5322 0.197
Cell Tubulin - Actin Correlation 0.074 876 of 5322 0.068
Cell DNA - Tubulin Correlation 0.070 1435 of 5322 0.064
Cell Shape Area -1.064e-01 4812 of 5322 0.009
Cell Shape Eccentricity -1.893e-02 4706 of 5322 0.873
Mitotic Score Rank FDR q-value
Nucleus DNA Integrated 0.006 3831 of 5322 0.997
Nucleus DNA Mean 0.036 2371 of 5322 1.000
Nucleus DNA Mass Displacement 0.072 2345 of 5322 0.999
Nucleus DNA Mean Frac 3 0.415 73 of 5322 1.000
Nucleus gH2AX Mean 0.015 2732 of 5322 1.000
Nucleus gH2AX Mean Frac 0 -4.119e-02 2950 of 5322 1.000
Nucleus Tubulin Mean Frac 0 -1.342e-01 4903 of 5322 0.999
Nucleus Tubulin Mean Edge 0.523 47 of 5322 0.268
Nucleus Tubulin Std Edge 0.344 106 of 5322 0.759
Nucleus Shape Area -1.598e-03 3962 of 5322 0.990
Nucleus Shape Eccentricity 0.105 811 of 5322 1.000
Nucleus Shape Form Factor -2.600e-01 4596 of 5322 0.976
Cell Tubulin Mean 0.484 107 of 5322 0.354
Cell Tubulin Mean Frac 3 -2.697e-01 4570 of 5322 0.999
Cell Actin Mean -2.391e-01 5140 of 5322 0.997
Cell Shape Area 0.115 2385 of 5322 0.998
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Day 3

Day 4

MIT Central Accessability